Application
A. ELISA assay using 5ug/mL Anti-AFP RabMAb (#1791-1) as the capture antibody and 0.5 ug/mL Anti-PEG-47-Biotin RabMAb (Cat. #AJ1639c) as the detection antibody. HRP-avidin (1:3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.Application
B. Sandwich ELISA assay using 5ug/mL Anti-GCSF as the capture antibody and 0.5 ug/mL Anti-PEG-47-Biotin RabMAb (Cat. #AJ1639c) as the detection antibody. HRP-avidin (1:3000) used for anti-PEG-47 detection. This ELISA assay could detect GCSF-PEG antigen at a concentration as low as 0.9 ng/mL.Application
C. Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1:3000) was used for the detection of varying amounts of Anit-PEG-47-Biotin (Cat. #AJ1639c).Application
D. Immunoblot of different antigen-PEG preparations. Western Blot images using Anti-PEG 47-Biotin (Cat. #AJ1639c) to detect BSA-PEG. Lane 1: 31.25 ng/mL; lane 2: 15.6 ng/mL; lane 3: 7.8 ng/mL.| Product Name | Polyethylene Glycol (PEG)-Biotin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | PEG-B-47b |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:15 ng/ml |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Tissue Specificity | KLH-PEG with terminal methoxy group was used as an immunogen. This antibody recognizes the terminal methoxy group of the PEG molecule. Anti-PEG-47-Biotin is prepared by the biotinylation of the antibody via primary amines. |
|---|
Application
A. ELISA assay using 5ug/mL Anti-AFP RabMAb (#1791-1) as the capture antibody and 0.5 ug/mL Anti-PEG-47-Biotin RabMAb (Cat. #AJ1639c) as the detection antibody. HRP-avidin (1:3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.
Application
B. Sandwich ELISA assay using 5ug/mL Anti-GCSF as the capture antibody and 0.5 ug/mL Anti-PEG-47-Biotin RabMAb (Cat. #AJ1639c) as the detection antibody. HRP-avidin (1:3000) used for anti-PEG-47 detection. This ELISA assay could detect GCSF-PEG antigen at a concentration as low as 0.9 ng/mL.
Application
C. Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1:3000) was used for the detection of varying amounts of Anit-PEG-47-Biotin (Cat. #AJ1639c).
Application
D. Immunoblot of different antigen-PEG preparations. Western Blot images using Anti-PEG 47-Biotin (Cat. #AJ1639c) to detect BSA-PEG. Lane 1: 31.25 ng/mL; lane 2: 15.6 ng/mL; lane 3: 7.8 ng/mL.| Application Notes | WB:15 ng/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
