Application
POLR3B Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the POLR3B antibody detected the POLR3B protein (arrow).| Product Name | POLR3B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR3B; DNA-directed RNA polymerase III subunit RPC2; C128; DNA-directed RNA polymerase III 127.6 kDa polypeptide; DNA-directed RNA polymerase III subunit B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POLR3B DNA-directed RNA polymerase III subunit RPC2 C128 DNA-directed RNA polymerase III 127.6 kDa polypeptide DNA-directed RNA polymerase III subunit B |
|---|---|
| Molecular Weight(MW) | 127785 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway |
| Tissue Specificity | This POLR3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-359 amino acids from the N-terminal region of human POLR3B. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 55703 |
|---|---|
| Nucleotide Accession | NP_060552.4 |
Application
POLR3B Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the POLR3B antibody detected the POLR3B protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
