Application
POLR3C Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the POLR3C antibody detected the POLR3C protein (arrow).Application
Western blot analysis of POLR3C (arrow) using rabbit polyclonal POLR3C Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR3C gene.| Product Name | POLR3C Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR3C; DNA-directed RNA polymerase III subunit RPC3; DNA-directed RNA polymerase III subunit C; RNA polymerase III 62 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POLR3C DNA-directed RNA polymerase III subunit RPC3 DNA-directed RNA polymerase III subunit C RNA polymerase III 62 kDa subunit |
|---|---|
| Molecular Weight(MW) | 60612 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB- DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway |
| Tissue Specificity | This POLR3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the Central region of human POLR3C. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 10623 |
|---|---|
| Nucleotide Accession | NP_006459.3 |
Application
POLR3C Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the POLR3C antibody detected the POLR3C protein (arrow).
Application
Western blot analysis of POLR3C (arrow) using rabbit polyclonal POLR3C Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR3C gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
