Application
POLR3G Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLR3G antibody detected the POLR3G protein (arrow).| Product Name | POLR3G Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR3G; DNA-directed RNA polymerase III subunit RPC7; DNA-directed RNA polymerase III subunit G; RNA polymerase III 32 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POLR3G DNA-directed RNA polymerase III subunit RPC7 DNA-directed RNA polymerase III subunit G RNA polymerase III 32 kDa subunit |
|---|---|
| Molecular Weight(MW) | 25914 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F- RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway |
| Tissue Specificity | This POLR3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-223 amino acids from the C-terminal region of human POLR3G. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 10622 |
|---|---|
| Nucleotide Accession | NP_006458.2 |
Application
POLR3G Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLR3G antibody detected the POLR3G protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
