Application
POMT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POMT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
POMT1 Antibody (C-term) western blot analysis in ZR-75-1,K562 cell line lysates (35ug/lane).This demonstrates the POMT1 antibody detected the POMT1 protein (arrow).Application
POMT1 Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | POMT1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POMT1; Protein O-mannosyl-transferase 1; Dolichyl-phosphate-mannose--protein mannosyltransferase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBFC |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POMT1 Protein O-mannosyl-transferase 1 Dolichyl-phosphate-mannose--protein mannosyltransferase 1 |
|---|---|
| Molecular Weight(MW) | 84881 Da |
| Function | Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient |
| Tissue Specificity | Widely expressed. Highly expressed in testis, heart and pancreas. Detected at lower levels in kidney, skeletal muscle, brain, placenta, lung and liver |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein |
| Entrez Gene | 10585 |
|---|---|
| Nucleotide Accession | NP_001070833.1 NP_001129586.1 |
Application
POMT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POMT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
POMT1 Antibody (C-term) western blot analysis in ZR-75-1,K562 cell line lysates (35ug/lane).This demonstrates the POMT1 antibody detected the POMT1 protein (arrow).
Application
POMT1 Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
