Application
Western blot analysis of PON1 (arrow) using rabbit polyclonal PON1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PON1 gene (Lane 2) .Application
PON1 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
PON1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PON1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PON1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PON1; PON; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; K-45; Serum aryldialkylphosphatase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIHC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PON |
|---|---|
| Alternative Names | PON1 PON Serum paraoxonase/arylesterase 1 Aromatic esterase 1 K-45 Serum aryldialkylphosphatase 1 |
| Molecular Weight(MW) | 39731 Da |
| Function | Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation |
| Tissue Specificity | Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas |
| Cellular Localization | Secreted, extracellular space. |
| Entrez Gene | 5444 |
|---|---|
| Nucleotide Accession | NP_000437.3 |
Application
Western blot analysis of PON1 (arrow) using rabbit polyclonal PON1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PON1 gene (Lane 2) .
Application
PON1 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
PON1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PON1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM218347