Application
Western blot analysis of POU2AF1 Antibody (N-term) in Ramos cell line lysates (35ug/lane). POU2AF1 (arrow) was detected using the purified Pab.Application
POU2AF1 Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
POU2AF1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POU2AF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | POU2AF1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POU2AF1; OBF1; POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIHC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OBF1 |
|---|---|
| Alternative Names | POU2AF1 OBF1 POU domain class 2-associating factor 1 B-cell-specific coactivator OBF-1 BOB-1 OCA-B OCT-binding factor 1 |
| Molecular Weight(MW) | 27436 Da |
| Function | Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers |
| Tissue Specificity | B-cell specific. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5450 |
|---|
Application
Western blot analysis of POU2AF1 Antibody (N-term) in Ramos cell line lysates (35ug/lane). POU2AF1 (arrow) was detected using the purified Pab.
Application
POU2AF1 Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
POU2AF1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POU2AF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
