Application
POSTN Antibody (C-term) western blot analysis in MCF-7(lane 1),A375(lane 2) cell line lysates (35ug/lane).This demonstrates the POSTN antibody detected the POSTN protein (arrow).Application
POSTN Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POSTN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of POSTN Antibody (C-term)(Cat#AP11962b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | POSTN Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POSTN; OSF2; Periostin; Osteoblast-specific factor 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OSF2 |
|---|---|
| Alternative Names | POSTN OSF2 Periostin Osteoblast-specific factor 2 |
| Molecular Weight(MW) | 93314 Da |
| Function | Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization |
| Tissue Specificity | Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers |
| Cellular Localization | Golgi apparatus (By similarity). Secreted, extracellular space, extracellular matrix. Note=Co-localizes with BMP1 in the Golgi (By similarity) |
Application
POSTN Antibody (C-term) western blot analysis in MCF-7(lane 1),A375(lane 2) cell line lysates (35ug/lane).This demonstrates the POSTN antibody detected the POSTN protein (arrow).
Application
POSTN Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POSTN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of POSTN Antibody (C-term)(Cat#AP11962b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
