Application
POU2F1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the POU2F1 antibody detected the POU2F1 protein (arrow).Application
POU2F1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POU2F1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | POU2F1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POU2F1; OCT1; OTF1; POU domain, class 2, transcription factor 1; NF-A1; Octamer-binding protein 1; Octamer-binding transcription factor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OCT1 OTF1 |
|---|---|
| Alternative Names | POU2F1 OCT1 OTF1 POU domain class 2 transcription factor 1 NF-A1 Octamer-binding protein 1 Octamer-binding transcription factor 1 |
| Molecular Weight(MW) | 76472 Da |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes |
| Tissue Specificity | Ubiquitous. Isoform 2 is lymphocyte-specific. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5451 |
|---|---|
| Nucleotide Accession | NP_002688.2 |
Application
POU2F1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the POU2F1 antibody detected the POU2F1 protein (arrow).
Application
POU2F1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POU2F1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
