Application
Figure 1: Western blot analysis using anti-SYK monoclonal antibody against truncated SYK-His recombinant protein(1) and PMA induced THP-1 cell lysate(2).| Product Name | SYK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ25043; FLJ37489; DKFZp313N1010 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8C1A3 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OCT1 |
|---|---|
| Alternative Names | FLJ25043 FLJ37489 DKFZp313N1010 |
| Molecular Weight(MW) | 61154 Da |
| Function | Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)- N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin- dependent kinase II and LCK tyrosine kinase |
| Tissue Specificity | Widely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines |
| Cellular Localization | Basolateral cell membrane; Multi-pass membrane protein |
| Entrez Gene | 6580 |
|---|---|
| Nucleotide Accession | NP_694857.1 |
Application
Figure 1: Western blot analysis using anti-SYK monoclonal antibody against truncated SYK-His recombinant protein(1) and PMA induced THP-1 cell lysate(2).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of SYK(aa296-484) expressed in E. Coli. |
