Application
A. Western blot analysis on Hela cell lysates using anti-Phospho-PP1 alpha (pT320) RabMAb (Cat. #AJ1642b). Cells were either (A) untreated or (B) treated with Calyculin A.Application
B. Immunohistochemical analysis of paraffin-embedded human muscles using anti-PP1alpha RabMAb (Cat. #AJ1642b).| Product Name | PP1 alpha Antibody Phospho (pT320) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPP1CA, PPP1A, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit |
| Modification | p-T320 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1512Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PPP1A |
|---|---|
| Alternative Names | PPP1CA PPP1A Serine/threonine-protein phosphatase PP1-alpha catalytic subunit |
| Molecular Weight(MW) | 37512 Da |
| Function | Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding Threonine 320 of human PP1 alpha was used as an immunogen. This antibody detects PP1 alpha phosphorylated at Threonine 320. |
| Cellular Localization | Cytoplasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles |
| Entrez Gene | 5499 |
|---|
Application
A. Western blot analysis on Hela cell lysates using anti-Phospho-PP1 alpha (pT320) RabMAb (Cat. #AJ1642b). Cells were either (A) untreated or (B) treated with Calyculin A.
Application
B. Immunohistochemical analysis of paraffin-embedded human muscles using anti-PP1alpha RabMAb (Cat. #AJ1642b).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
