Application
Western blot analysis of anti-PPP1CA Antibody (C-term) in mouse heart tissue lysate (35ug/lane). PPP1CA(arrow) was detected using the purified Pab.Application
Western blot analysis of anti-PPP1CA Antibody (C-term) (RB05936) in Jurkat cell line lysates (35ug/lane). PPP1CA(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PPP1A (PPP1CA) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPP1CA; PPP1A; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PPP1A |
|---|---|
| Alternative Names | PPP1CA PPP1A Serine/threonine-protein phosphatase PP1-alpha catalytic subunit |
| Molecular Weight(MW) | 37512 Da |
| Function | Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development |
| Tissue Specificity | This PPP1A (PPP1CA) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293~323 amino acids from the C-terminal region of human PPP1CA. |
| Cellular Localization | Cytoplasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles |
| Entrez Gene | 5499 |
|---|
Application
Western blot analysis of anti-PPP1CA Antibody (C-term) in mouse heart tissue lysate (35ug/lane). PPP1CA(arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-PPP1CA Antibody (C-term) (RB05936) in Jurkat cell line lysates (35ug/lane). PPP1CA(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
