Application
A. Western blot analysis on A: A431 cell lysate B: NIH 3T3 cell lysate using anti-PP2A (N-term) RabMAb (Cat. #AJ1644b), dilution 1:500.Application
B. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-PP2A (N-term) RabMAb (Cat. #AJ1644b).| Product Name | PP2A Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPP2CA, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, Replication protein C |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | Cocktail |
| Clone Number | YE351 |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500 IHC |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | PPP2CA Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Replication protein C |
|---|---|
| Molecular Weight(MW) | 35594 Da |
| Function | PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259' |
| Tissue Specificity | A synthetic peptide corresponding to residues in N-terminus of human PP2A-alpha was used as immunogen. Predicted to cross-react with bovin and pig, based on sequence homology. |
| Cellular Localization | Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity) |
| Entrez Gene | 5515 |
|---|
Application
A. Western blot analysis on A: A431 cell lysate B: NIH 3T3 cell lysate using anti-PP2A (N-term) RabMAb (Cat. #AJ1644b), dilution 1:500.
Application
B. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-PP2A (N-term) RabMAb (Cat. #AJ1644b).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
