Application
A. Western blot analysis on A431 cell lysate using anti-Phospho-PP2A (Y307) RabMAb (Cat. #AJ1644c), dilution 1:5,000. Cell lysates were either (1) untreated or (2) treated with EGF.Application
B. Immunohistochemical analysis of paraffin-embedded human brain using anti-Phospho-PP2A (Y307) RabMAb (Cat. #AJ1644c).| Product Name | PP2A Antibody Phospho (pY307) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPP2CA, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, Replication protein C |
| Modification | p-Y307 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E155 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PPP2CA Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Replication protein C |
|---|---|
| Molecular Weight(MW) | 35594 Da |
| Function | PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259' |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Tyr307 of human PP2A was used as immunogen. The antibody only detects PP2A phosphorylated on Tyrosine 307. Predicted to cross-react with mouse, rat, bovine and pig, based on sequence homology. |
| Cellular Localization | Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity) |
| Entrez Gene | 5515 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-Phospho-PP2A (Y307) RabMAb (Cat. #AJ1644c), dilution 1:5,000. Cell lysates were either (1) untreated or (2) treated with EGF.
Application
B. Immunohistochemical analysis of paraffin-embedded human brain using anti-Phospho-PP2A (Y307) RabMAb (Cat. #AJ1644c).| Application Notes | WB:1:10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
