Application
Western blot analysis of PPIA Antibody (N-term) in T47D cell line(lane 1) and mouse brain(lane 2),bladder(lane 3) tissue lysates (35ug/lane). PPIA (arrow) was detected using the purified Pab.(2ug/ml)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PPIA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of widr cells using PPIA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PPIA Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPIA; CYPA; Peptidyl-prolyl cis-trans isomerase A; Cyclophilin A; Cyclosporin A-binding protein; Rotamase A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYPA |
|---|---|
| Alternative Names | PPIA CYPA Peptidyl-prolyl cis-trans isomerase A Cyclophilin A Cyclosporin A-binding protein Rotamase A |
| Molecular Weight(MW) | 18012 Da |
| Function | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides |
| Tissue Specificity | This PPIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~45 amino acids from the N-terminal region of human PPIA. |
| Cellular Localization | Cytoplasm. Secreted. Note=Secretion occurs in response to oxidative stress in vascular smooth muscle through a vesicular secretory pathway that involves actin remodeling and myosin II activation, and mediates ERK1/2 activation |
| Entrez Gene | 5478 |
|---|
Application
Western blot analysis of PPIA Antibody (N-term) in T47D cell line(lane 1) and mouse brain(lane 2),bladder(lane 3) tissue lysates (35ug/lane). PPIA (arrow) was detected using the purified Pab.(2ug/ml)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PPIA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of widr cells using PPIA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
