WB
Various lysates were subjected to SDS PAGE followed by western blot with CYPA antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody as loading control.ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cyclophilin A antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin A and Omnimabs® 488 Goat Anti-Mouse IgG(H&L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | Cyclophilin A Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CYPA protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFWB |
| WB:1:5000-1:50000 ICC/IF:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPIA |
|---|---|
| Gene Synonyms | CYPA CYPH HEL-S-69p |
| Gene Full Name | peptidylprolyl isomerase A |
| Gene Summary | This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008] |
| Alternative Names | PPIA 2B11B9 Cyclosporin A binding protein Cyclosporin A-binding protein CYPA |
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Cytoplasm,Nucleus,Secreted. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with CYPA antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody as loading control.
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cyclophilin A antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).
FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin A and Omnimabs® 488 Goat Anti-Mouse IgG(H&L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:5000-1:50000 ICC/IF:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
