Application
PPIF Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPIF antibody detected the PPIF protein (arrow).| Product Name | PPIF Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPIF; CYP3; Peptidyl-prolyl cis-trans isomerase F, mitochondrial; Cyclophilin F; Rotamase F |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYP3 |
|---|---|
| Alternative Names | PPIF CYP3 Peptidyl-prolyl cis-trans isomerase F mitochondrial Cyclophilin F Rotamase F |
| Molecular Weight(MW) | 22040 Da |
| Function | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probablity of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress- induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis |
| Tissue Specificity | This PPIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-131 amino acids from the Central region of human PPIF. |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 10105 |
|---|---|
| Nucleotide Accession | NP_005720.1 |
Application
PPIF Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPIF antibody detected the PPIF protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
