Application
Western blot analysis of anti-PPM1F Pab in mouse liver tissue lysate (35ug/lane). PPM1F(arrow) was detected using the purified Pab.Application
Western blot analysis of PPM1F (arrow) using PPM1F Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PPM1F Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPM1F; KIAA0015; POPX2; Protein phosphatase 1F; Ca(2+)/calmodulin-dependent protein kinase phosphatase; Partner of PIX 2; Protein fem-2 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0015 POPX2 |
|---|---|
| Alternative Names | PPM1F KIAA0015 POPX2 Protein phosphatase 1F Ca(2+)/calmodulin-dependent protein kinase phosphatase Partner of PIX 2 Protein fem-2 homolog |
| Molecular Weight(MW) | 49831 Da |
| Function | Dephosphorylates and concomitantly deactivates CaM- kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase Promotes apoptosis |
| Tissue Specificity | This PPM1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18~48 amino acids from the N-terminal region of human PPM1F. |
| Entrez Gene | 9647 |
|---|
Application
Western blot analysis of anti-PPM1F Pab in mouse liver tissue lysate (35ug/lane). PPM1F(arrow) was detected using the purified Pab.
Application
Western blot analysis of PPM1F (arrow) using PPM1F Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
