Application
Western blot analysis of PPP2R1A Antibody (C-term) (Cat.# AP1934b) in NIH-3T3 cell line lysates (35ug/lane). PPP2R1A (arrow) was detected using the purified Pab.| Product Name | PPP2R1A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPP2R1A; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PPP2R1A Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Medium tumor antigen-associated 61 kDa protein PP2A subunit A isoform PR65-alpha PP2A subunit A isoform R1-alpha |
|---|---|
| Molecular Weight(MW) | 65309 Da |
| Function | The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis |
| Tissue Specificity | This PPP2R1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 459~489 amino acids from the C-terminal region of human PPP2R1A. |
| Cellular Localization | Cytoplasm (By similarity). Chromosome, centromere. Note=Centromeric localization requires the presence of BUB1 |
Application
Western blot analysis of PPP2R1A Antibody (C-term) (Cat.# AP1934b) in NIH-3T3 cell line lysates (35ug/lane). PPP2R1A (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
