Application
Western blot analysis of PPP2R2B Antibody (Center) in Hela cell line lysates (35ug/lane). PPP2R2B (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue with PPP2R2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of hela cells using PPP2R2B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PPP2R2B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPP2R2B; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform; PP2A subunit B isoform B55-beta; PP2A subunit B isoform PR55-beta; PP2A subunit B isoform R2-beta; PP2A subunit B isoform beta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PPP2R2B Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform PP2A subunit B isoform B55-beta PP2A subunit B isoform PR55-beta PP2A subunit B isoform R2-beta PP2A subunit B isoform beta |
|---|---|
| Molecular Weight(MW) | 51710 Da |
| Function | The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity (By similarity). Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance (By similarity) |
| Tissue Specificity | Brain. |
| Cellular Localization | Isoform 1: Cytoplasm (By similarity). Cytoplasm, cytoskeleton (By similarity). Membrane (By similarity) |
| Entrez Gene | 5521 |
|---|
Application
Western blot analysis of PPP2R2B Antibody (Center) in Hela cell line lysates (35ug/lane). PPP2R2B (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue with PPP2R2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of hela cells using PPP2R2B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
