Application
PPP4R2 Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the PPP4R2 antibody detected the PPP4R2 protein (arrow).| Product Name | PPP4R2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPP4R2; Serine/threonine-protein phosphatase 4 regulatory subunit 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PPP4R2 Serine/threonine-protein phosphatase 4 regulatory subunit 2 |
|---|---|
| Molecular Weight(MW) | 46898 Da |
| Function | Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Nucleus. Note=Ionizing radiation induces relocalization to nuclear foci and colocalization with RPA2 |
| Entrez Gene | 151987 |
|---|---|
| Nucleotide Accession | NP_777567.1 |
Application
PPP4R2 Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the PPP4R2 antibody detected the PPP4R2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
