Application
Figure 1: Western blot analysis using anti-PRAK monoclonal antiobdy against truncated PRAK recombinant protein.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma(A), esophagus carcinoma(B), normal spleen tissue(C), breast carcinoma(D), showing nuclear and cytoplasmic localization using anti-PRAK antibody with DAB staining.| Product Name | PRAK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRAK; MAPKAPK5 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7H10B4 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PRAK |
|---|---|
| Alternative Names | PRAK MAPKAPK5 |
| Molecular Weight(MW) | 54220 Da |
| Function | Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras- induced senescence and phosphorylating p53/TP53. Involved in post- transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement |
| Tissue Specificity | Expressed ubiquitously. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export |
| Entrez Gene | 8550 |
|---|---|
| Nucleotide Accession | NP_620777.1 |
Application
Figure 1: Western blot analysis using anti-PRAK monoclonal antiobdy against truncated PRAK recombinant protein.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma(A), esophagus carcinoma(B), normal spleen tissue(C), breast carcinoma(D), showing nuclear and cytoplasmic localization using anti-PRAK antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of PRAK expressed in E. Coli. |
