Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-PRC1 (pT481) RabMAb (Cat. #AJ1648b), 1:500 dilution. Cells were either (A) untreated (B) treated with aphidicolin + nocodazole.Application
B. Immunofluorescent staining of HeLa cells using anti-PRC1 RabMAb (Cat. #AJ1648b).| Product Name | PRC1 Antibody Phospho (pT481) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRC1, Protein regulator of cytokinesis 1 |
| Modification | p-T481 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1514Y |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PRC1 Protein regulator of cytokinesis 1 |
|---|---|
| Molecular Weight(MW) | 71607 Da |
| Function | Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding Threonine 481 of human PRC1 was used as an immunogen. This antibody detects PRC1 phosphorylated at Theonine 481. |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Note=Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis |
| Entrez Gene | 9055 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-PRC1 (pT481) RabMAb (Cat. #AJ1648b), 1:500 dilution. Cells were either (A) untreated (B) treated with aphidicolin + nocodazole.
Application
B. Immunofluorescent staining of HeLa cells using anti-PRC1 RabMAb (Cat. #AJ1648b).| Application Notes | WB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
