Application
Western blot analysis of PRAMEF3 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in NCI-H460 cell line lysate. PRAMEF3 Antibody (C-term) (arrow) was detected using the purified Pab (1:60/250 dilution).Application
PRAMEF3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRAMEF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PRAMEF3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRAMEF3; PRAME family member 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PRAMEF3 PRAME family member 3 |
|---|---|
| Molecular Weight(MW) | 55198 Da |
| Tissue Specificity | This PRAMEF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 427-457 amino acids from the C-terminal region of human PRAMEF3. |
Application
Western blot analysis of PRAMEF3 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in NCI-H460 cell line lysate. PRAMEF3 Antibody (C-term) (arrow) was detected using the purified Pab (1:60/250 dilution).
Application
PRAMEF3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRAMEF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
