Application
Western blot analysis of PRC1 antibody (Center H439) in mouse testis tissue lysates (35ug/lane). PRC1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded mouse testis with PRC1 Antibody (Center H439), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of widr cells using PRC1 Antibody (Center H439)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PRC1 (arrow) using rabbit polyclonal PRC1 Antibody (Center H439) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRC1 gene.| Product Name | PRC1 Antibody (Center H439) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRC1; Protein regulator of cytokinesis 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PRC1 Protein regulator of cytokinesis 1 |
|---|---|
| Molecular Weight(MW) | 71607 Da |
| Function | Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle |
| Tissue Specificity | This PRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 431~461 amino acids from the Center region of human PRC1. |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Note=Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis |
| Entrez Gene | 9055 |
|---|
Application
Western blot analysis of PRC1 antibody (Center H439) in mouse testis tissue lysates (35ug/lane). PRC1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded mouse testis with PRC1 Antibody (Center H439), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of widr cells using PRC1 Antibody (Center H439)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PRC1 (arrow) using rabbit polyclonal PRC1 Antibody (Center H439) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRC1 gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
