Application
Western blot analysis of PRDX1 Antibody (Center) in CEM cell line lysates (35ug/lane). PRDX1 (arrow) was detected using the purified Pab.Application
Western blot analysis of PRDX1 (arrow) using rabbit polyclonal PRDX1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX1 gene (Lane 2) .Application
PRDX1 Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
PRDX1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRDX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PRDX1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRDX1; PAGA; PAGB; TDPX2; Peroxiredoxin-1; Natural killer cell-enhancing factor A; Proliferation-associated gene protein; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIHC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PAGA PAGB TDPX2 |
|---|---|
| Alternative Names | PRDX1 PAGA PAGB TDPX2 Peroxiredoxin-1 Natural killer cell-enhancing factor A Proliferation-associated gene protein Thioredoxin peroxidase 2 Thioredoxin-dependent peroxide reductase 2 |
| Molecular Weight(MW) | 22110 Da |
| Function | Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity) |
| Tissue Specificity | This PRDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95~124 amino acids from the Center region of human PRDX1. |
| Cellular Localization | Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 5052 |
|---|
Application
Western blot analysis of PRDX1 Antibody (Center) in CEM cell line lysates (35ug/lane). PRDX1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PRDX1 (arrow) using rabbit polyclonal PRDX1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX1 gene (Lane 2) .
Application
PRDX1 Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
PRDX1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRDX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
