Application
Western blot analysis of PRDX3 Antibody (N-term) in NCI-H460,MDA-MB231,HepG2 cell line lysates (35ug/lane). PRDX3 (arrow) was detected using the purified Pab.(2ug/ml)Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRDX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-MB468 cells using PRDX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PRDX3 (arrow) using rabbit polyclonal PRDX3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX3 gene.| Product Name | PRDX3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRDX3; AOP1; Thioredoxin-dependent peroxide reductase, mitochondrial; Antioxidant protein 1; HBC189; Peroxiredoxin III; Peroxiredoxin-3; Protein MER5 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AOP1 |
|---|---|
| Alternative Names | PRDX3 AOP1 Thioredoxin-dependent peroxide reductase mitochondrial Antioxidant protein 1 HBC189 Peroxiredoxin III Peroxiredoxin-3 Protein MER5 homolog |
| Molecular Weight(MW) | 27693 Da |
| Function | Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol |
| Tissue Specificity | This PRDX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73~102 amino acids from the N-terminal region of human PRDX3. |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 10935 |
|---|
Application
Western blot analysis of PRDX3 Antibody (N-term) in NCI-H460,MDA-MB231,HepG2 cell line lysates (35ug/lane). PRDX3 (arrow) was detected using the purified Pab.(2ug/ml)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRDX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-MB468 cells using PRDX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PRDX3 (arrow) using rabbit polyclonal PRDX3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX3 gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
