Application
A. Western blot analysis on 293T cell lysate using anti-PRK2 RabMAb (Cat. #AJ1653a), dilution 1:1,000.Application
B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-PRK2 RabMAb (Cat. #AJ1653a).| Product Name | PRK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKN2, PRK2, PRKCL2, Serine/threonine-protein kinase N2, Protein kinase C-like 2;Protein-kinase C-related kinase 2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E352 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PRK2 PRKCL2 |
|---|---|
| Alternative Names | PKN2 PRK2 PRKCL2 Serine/threonine-protein kinase N2 Protein kinase C-like 2 Protein-kinase C-related kinase 2 |
| Molecular Weight(MW) | 112035 Da |
| Function | PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity Mediates Rho protein-induced transcriptional activation via the c- fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication |
| Tissue Specificity | Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells |
| Cellular Localization | Cytoplasm. Nucleus. Membrane (By similarity). Cell projection, lamellipodium. Cytoplasm, cytoskeleton. Cleavage furrow. Midbody. Cell junction Note=Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm |
| Entrez Gene | 5586 |
|---|
Application
A. Western blot analysis on 293T cell lysate using anti-PRK2 RabMAb (Cat. #AJ1653a), dilution 1:1,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-PRK2 RabMAb (Cat. #AJ1653a).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
