Application
PRKAG3 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PRKAG3 antibody detected the PRKAG3 protein (arrow).| Product Name | PRKAG3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAG3; AMPKG3; 5'-AMP-activated protein kinase subunit gamma-3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AMPKG3 |
|---|---|
| Alternative Names | PRKAG3 AMPKG3 5'-AMP-activated protein kinase subunit gamma-3 |
| Molecular Weight(MW) | 54258 Da |
| Function | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive |
| Tissue Specificity | Skeletal muscle, with weak expression in heart and pancreas |
| Entrez Gene | 53632 |
|---|---|
| Nucleotide Accession | NP_059127.2 |
Application
PRKAG3 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PRKAG3 antibody detected the PRKAG3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
