Application
Western blot analysis of anti-PRKRA Antibody (S246) in HL60 cell line lysates (35ug/lane). PRKRA (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human placenta reacted with PRKRA Antibody (S246), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
PRKRA Antibody (S246) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PRKRA Antibody (S246) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKRA; PACT; RAX; Interferon-inducible double stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; Protein kinase, interferon-inducible double s |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PACT RAX |
|---|---|
| Alternative Names | PRKRA PACT RAX Interferon-inducible double stranded RNA-dependent protein kinase activator A PKR-associated protein X PKR-associating protein X Protein activator of the interferon-induced protein kinase Protein kinase interferon-inducible double s |
| Molecular Weight(MW) | 34404 Da |
| Function | Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post- transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1 |
| Tissue Specificity | This PRKRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231~261 amino acids from S246 region of human PRKRA. |
| Cellular Localization | Cytoplasm, perinuclear region. |
| Entrez Gene | 8575 |
|---|
Application
Western blot analysis of anti-PRKRA Antibody (S246) in HL60 cell line lysates (35ug/lane). PRKRA (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human placenta reacted with PRKRA Antibody (S246), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
PRKRA Antibody (S246) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
