Application
Western blot analysis on (A) A549 (B)HeLa and (C) NIH/3T3 cell lysates using anti-PRMT1 RabMAb (Cat. #AJ1654a).Application
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PRMT1 RabMAb (Cat. #AJ1654a).| Product Name | PRMT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRMT1, HMT2, HRMT1L2, IR1B4, Protein arginine N-methyltransferase 1, Interferon receptor 1-bound protein 4 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3292 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HMT2 HRMT1L2 IR1B4 |
|---|---|
| Alternative Names | PRMT1 HMT2 HRMT1L2 IR1B4 Protein arginine N-methyltransferase 1 Interferon receptor 1-bound protein 4 |
| Molecular Weight(MW) | 41516 Da |
| Function | Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway Methylates FOXO1 and retains it in the nucleus increasing its transcriptional acivity |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Nucleus. Nucleus, nucleoplasm (By similarity). Cytoplasm, cytosol (By similarity). Note=Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity) |
| Entrez Gene | 3276 |
|---|
Application
Western blot analysis on (A) A549 (B)HeLa and (C) NIH/3T3 cell lysates using anti-PRMT1 RabMAb (Cat. #AJ1654a).
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PRMT1 RabMAb (Cat. #AJ1654a).| Application Notes | WB:1:5000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
