Application
Western blot analysis of PRMT1 (arrow) using rabbit polyclonal PRMT1 Antibody (C-term) (RB00005). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PRMT1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRMT1; HMT2; HRMT1L2; IR1B4; Protein arginine N-methyltransferase 1; Histone-arginine N-methyltransferase PRMT1; Interferon receptor 1-bound protein 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HMT2 HRMT1L2 IR1B4 |
|---|---|
| Alternative Names | PRMT1 HMT2 HRMT1L2 IR1B4 Protein arginine N-methyltransferase 1 Histone-arginine N-methyltransferase PRMT1 Interferon receptor 1-bound protein 4 |
| Molecular Weight(MW) | 41516 Da |
| Function | Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway Methylates FOXO1 and retains it in the nucleus increasing its transcriptional acivity |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Nucleus. Nucleus, nucleoplasm (By similarity). Cytoplasm, cytosol (By similarity). Note=Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity) |
| Entrez Gene | 3276 |
|---|
Application
Western blot analysis of PRMT1 (arrow) using rabbit polyclonal PRMT1 Antibody (C-term) (RB00005). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
