Application
A. Western blot analysis on (A) MCF-7, (B) PC-12 (C) A431 and (D) Ramos cell lysate using anti-prohibitin RabMAb (Cat. #AJ1656a), dilution 1:1000.Application
B. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Prohibitin.Application
C. Immunofluorescent staining of HeLa cell using anti-Prohibitin RabMAb (Cat. #AJ1656a).| Product Name | Prohibitin Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHB, Prohibitin |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2804Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PHB Prohibitin |
|---|---|
| Molecular Weight(MW) | 29804 Da |
| Function | Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging |
| Tissue Specificity | Widely expressed in different tissues. |
| Cellular Localization | Mitochondrion inner membrane (By similarity) |
| Entrez Gene | 5245 |
|---|
Application
A. Western blot analysis on (A) MCF-7, (B) PC-12 (C) A431 and (D) Ramos cell lysate using anti-prohibitin RabMAb (Cat. #AJ1656a), dilution 1:1000.
Application
B. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Prohibitin.
Application
C. Immunofluorescent staining of HeLa cell using anti-Prohibitin RabMAb (Cat. #AJ1656a).| Application Notes | WB:1:1000~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
