Application
Western blot analysis of anti-PKR1 Antibody (N-term) in CEM cell line lysates (35ug/lane). PKR1 (arrow) was detected using the purified Pab.Application
Western blot analysis of PKR1 (arrow) using rabbit polyclonal hPKR1-M1 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKR1 gene (Lane 2).(2ug/ml)Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Protein Kinase A regulatory subunit I alpha Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAR1A; PKR1; PRKAR1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PKR1 PRKAR1 TSE1 |
|---|---|
| Alternative Names | PRKAR1A PKR1 PRKAR1 TSE1 cAMP-dependent protein kinase type I-alpha regulatory subunit Tissue-specific extinguisher 1 |
| Molecular Weight(MW) | 42982 Da |
| Function | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells |
| Tissue Specificity | Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible |
| Cellular Localization | Cell membrane. |
| Entrez Gene | 5573 |
|---|
Application
Western blot analysis of anti-PKR1 Antibody (N-term) in CEM cell line lysates (35ug/lane). PKR1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PKR1 (arrow) using rabbit polyclonal hPKR1-M1 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKR1 gene (Lane 2).(2ug/ml)
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
