Application
Western blot analysis of PRPF19 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). PRPF19 (arrow) was detected using the purified Pab.Application
Western blot analysis of PRPF19 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). PRPF19 (arrow) was detected using the purified Pab.Application
PRPF19 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRPF19 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PRPF19 Antibody (N-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PRPF19 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRPF19; NMP200; PRP19; SNEV; Pre-mRNA-processing factor 19; Nuclear matrix protein 200; PRP19/PSO4 homolog; Senescence evasion factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NMP200 PRP19 SNEV |
|---|---|
| Alternative Names | PRPF19 NMP200 PRP19 SNEV Pre-mRNA-processing factor 19 Nuclear matrix protein 200 PRP19/PSO4 homolog Senescence evasion factor |
| Molecular Weight(MW) | 55181 Da |
| Function | Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing |
| Tissue Specificity | Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines |
| Cellular Localization | Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, spindle. Note=Nucleoplasmic in interphase cells Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin |
| Entrez Gene | 27339 |
|---|
Application
Western blot analysis of PRPF19 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). PRPF19 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PRPF19 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). PRPF19 (arrow) was detected using the purified Pab.
Application
PRPF19 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRPF19 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PRPF19 Antibody (N-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
