Application
Western blot analysis of PRUNE Antibody (C-term) in Jurkat cell line lysates (35ug/lane). PRUNE (arrow) was detected using the purified Pab.Application
PRUNE Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRUNE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PRUNE Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PRUNE Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRUNE; Protein prune homolog; Drosophila-related expressed sequence 17; HTcD37 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PRUNE Protein prune homolog Drosophila-related expressed sequence 17 HTcD37 |
|---|---|
| Molecular Weight(MW) | 50200 Da |
| Function | Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1 |
| Tissue Specificity | Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas |
| Cellular Localization | Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed |
| Entrez Gene | 58497 |
|---|
Application
Western blot analysis of PRUNE Antibody (C-term) in Jurkat cell line lysates (35ug/lane). PRUNE (arrow) was detected using the purified Pab.
Application
PRUNE Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRUNE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PRUNE Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
