Application
A. Western blot analysis on LnCaP cell lysate using anti-PSMA RabMAb (Cat. #AJ1663a), dilution 1:5000.| Product Name | PSMA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOLH1, FOLH, NAALAD1, PSM, PSMA, Glutamate carboxypeptidase 2, Cell growth-inhibiting protein 27 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP3253 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FOLH NAALAD1 PSM PSMA |
|---|---|
| Alternative Names | FOLH1 FOLH NAALAD1 PSM PSMA Glutamate carboxypeptidase 2 Cell growth-inhibiting protein 27 |
| Molecular Weight(MW) | 84331 Da |
| Function | Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression |
| Tissue Specificity | Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein |
| Entrez Gene | 2346 |
|---|
Application
A. Western blot analysis on LnCaP cell lysate using anti-PSMA RabMAb (Cat. #AJ1663a), dilution 1:5000.| Application Notes | WB:1:1000~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
