Application
The anti-PSKH1 Pab is used in Western blot to detect PSKH1 in mouse lung tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of PSKH1 (arrow) using rabbit polyclonal PSKH1 Antibody (T67) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSKH1 gene.Application
PSKH1 Antibody (T67) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the PSKH1 antibody detected the PSKH1 protein (arrow).| Product Name | PSKH1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSKH1; Serine/threonine-protein kinase H1; Protein serine kinase H1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PSKH1 Serine/threonine-protein kinase H1 Protein serine kinase H1 |
|---|---|
| Molecular Weight(MW) | 48035 Da |
| Function | May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing |
| Tissue Specificity | Expressed in all tissues and cell lines tested with the highest level of abundance in testis |
| Cellular Localization | Golgi apparatus. Cytoplasm, cytoskeleton, centrosome. Nucleus speckle. Endoplasmic reticulum membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Cytoplasm Note=Localized in the brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes Dual acylation is required to stabilize the interaction with Golgi membranes |
| Entrez Gene | 5681 |
|---|
Application
The anti-PSKH1 Pab is used in Western blot to detect PSKH1 in mouse lung tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of PSKH1 (arrow) using rabbit polyclonal PSKH1 Antibody (T67) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSKH1 gene.
Application
PSKH1 Antibody (T67) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the PSKH1 antibody detected the PSKH1 protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
