Application
Western blot analysis of PSMA6 Antibody (Center) in MCF-7, Ramos cell line lysates (35ug/lane). PSMA6 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PSMA6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
PSMA6 Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PSMA6 (arrow) using rabbit polyclonal PSMA6 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSMA6 gene.| Product Name | PSMA6 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSMA6; PROS27; Proteasome subunit alpha type-6; 27 kDa prosomal protein; Macropain iota chain; Multicatalytic endopeptidase complex iota chain; Proteasome iota chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PROS27 |
|---|---|
| Alternative Names | PSMA6 PROS27 Proteasome subunit alpha type-6 27 kDa prosomal protein Macropain iota chain Multicatalytic endopeptidase complex iota chain Proteasome iota chain |
| Molecular Weight(MW) | 27399 Da |
| Function | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity |
| Tissue Specificity | This PSMA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77~106 amino acids from the Center region of human PSMA6. |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, P-body (By similarity). Note=Co-localizes with TRIM5 in the cytoplasmic bodies (By similarity) |
| Entrez Gene | 5687 |
|---|
Application
Western blot analysis of PSMA6 Antibody (Center) in MCF-7, Ramos cell line lysates (35ug/lane). PSMA6 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PSMA6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
PSMA6 Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PSMA6 (arrow) using rabbit polyclonal PSMA6 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSMA6 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
