Application
Western blot analysis of PSMB6 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). PSMB6 (arrow) was detected using the purified Pab.(2ug/ml)Application
Western blot analysis of PSMB6 (arrow) using rabbit polyclonal PSMB6 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMB6 gene (Lane 2).(2ug/ml)Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with PSMB6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
PSMB6 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PSMB6 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSMB6; LMPY; Y; Proteasome subunit beta type-6; Macropain delta chain; Multicatalytic endopeptidase complex delta chain; Proteasome delta chain; Proteasome subunit Y |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LMPY Y |
|---|---|
| Alternative Names | PSMB6 LMPY Y Proteasome subunit beta type-6 Macropain delta chain Multicatalytic endopeptidase complex delta chain Proteasome delta chain Proteasome subunit Y |
| Molecular Weight(MW) | 25358 Da |
| Function | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens |
| Tissue Specificity | This PSMB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164~194 amino acids from the C-terminal region of human PSMB6. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 5694 |
|---|
Application
Western blot analysis of PSMB6 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). PSMB6 (arrow) was detected using the purified Pab.(2ug/ml)
Application
Western blot analysis of PSMB6 (arrow) using rabbit polyclonal PSMB6 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMB6 gene (Lane 2).(2ug/ml)
Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with PSMB6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
PSMB6 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
