Application
PSMD10 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PSMD10 antibody detected the PSMD10 protein (arrow).Application
Western blot analysis of PSMD10 (arrow) using rabbit polyclonal PSMD10 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSMD10 gene.| Product Name | PSMD10 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSMD10; 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; Gankyrin; p28(GANK) |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PSMD10 26S proteasome non-ATPase regulatory subunit 10 26S proteasome regulatory subunit p28 Gankyrin p28(GANK) |
|---|---|
| Molecular Weight(MW) | 24428 Da |
| Function | Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC) In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis |
| Tissue Specificity | Tends to be up-regulated in cancer cells with RAS mutations, including lung cancers and adenocarconimas (at protein level) |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 5716 |
|---|---|
| Nucleotide Accession | NP_002805.1 NP_736606.1 |
Application
PSMD10 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PSMD10 antibody detected the PSMD10 protein (arrow).
Application
Western blot analysis of PSMD10 (arrow) using rabbit polyclonal PSMD10 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSMD10 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
