Application
PSTPIP1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PSTPIP1 antibody detected the PSTPIP1 protein (arrow).Application
PSTPIP1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PSTPIP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSTPIP1; CD2BP1; Proline-serine-threonine phosphatase-interacting protein 1; CD2-binding protein 1; H-PIP |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD2BP1 |
|---|---|
| Alternative Names | PSTPIP1 CD2BP1 Proline-serine-threonine phosphatase-interacting protein 1 CD2-binding protein 1 H-PIP |
| Molecular Weight(MW) | 47591 Da |
| Function | Involved in regulation of the actin cytoskeleton. May regulate the WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to the ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allows PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation (By similarity). Down-regulates CD2- stimulated adhesion through the coupling of PTPN12 to CD2 |
| Tissue Specificity | Highly expressed in the peripheral blood leukocytes, granulocytes and monocytes, namely in T-cells and natural killer cells, and in spleen. Weakly expressed in the thymus, small intestine, lung and placenta |
| Cellular Localization | Cytoplasm (By similarity). Cytoplasm, cytoskeleton (By similarity). Cell projection, lamellipodium (By similarity). Cytoplasm, perinuclear region (By similarity) Cleavage furrow (By similarity). Note=Colocalized with the cortical actin cytoskeleton during interphase, lamellipodia and actin-rich cytokinetic cleavage furrow. Colocalized with WAS to filamentous structures within the cytoplasm. Colocalized with PTPN12 in the cytoplasm and the perinuclear region. Colocalized with CD2AP and WAS in the actin cytoskeleton. Colocalized with CD2, CD2AP and WAS at the site of T-cell:APC contact (By similarity) |
| Entrez Gene | 9051 |
|---|---|
| Nucleotide Accession | NP_003969.2 |
Application
PSTPIP1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PSTPIP1 antibody detected the PSTPIP1 protein (arrow).
Application
PSTPIP1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
