Application
Western blot analysis of PTHLH antibody (Center) in mouse spleen tissue lysates (35ug/lane). PTHLH (arrow) was detected using the purified Pab.Application
PTHLH Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PTHLH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PTHLH Antibody (Center) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PTHLH Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTHLH; PTHRP; Parathyroid hormone-related protein; Parathyroid hormone-like protein; PTHrP[1-36]; PTHrP[38-94]; Osteostatin; PTHrP[107-139] |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTHRP |
|---|---|
| Alternative Names | PTHLH PTHRP Parathyroid hormone-related protein Parathyroid hormone-like protein PTHrP[1-36] PTHrP[38-94] Osteostatin PTHrP[107-139] |
| Molecular Weight(MW) | 20194 Da |
| Function | Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath (By similarity). Promotes colon cancer cell migration and invasion in an integrin alpha-6/beta-1- dependent manner through activation of Rac1 |
| Tissue Specificity | Ubiquitous. Also expressed in the mammary gland |
| Cellular Localization | Cytoplasm. Nucleus. Secreted. |
| Entrez Gene | 5744 |
|---|
Application
Western blot analysis of PTHLH antibody (Center) in mouse spleen tissue lysates (35ug/lane). PTHLH (arrow) was detected using the purified Pab.
Application
PTHLH Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PTHLH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PTHLH Antibody (Center) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
