Application
PTN Antibody (C-term) western blot analysis in U251,NCI-H460 cell line lysates (35ug/lane).This demonstrates the PTN antibody detected the PTN protein (arrow).Application
PTN Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PTN Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PTN Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HBNF1, NEGF1 |
|---|---|
| Alternative Names | PTN HBNF1 NEGF1 Pleiotrophin Heparin-binding brain mitogen Heparin-binding growth factor 8 Heparin-binding growth-associated molecule Heparin-binding neurite outgrowth-promoting factor 1 Osteoblast-specific factor 1 |
| Molecular Weight(MW) | 18942 Da |
| Function | Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti- apoptotic signaling of PTN and regulation of cell proliferation |
| Tissue Specificity | Osteoblast and brain. |
| Cellular Localization | Secreted. |
| Entrez Gene | 5764 |
|---|---|
| Nucleotide Accession | NP_002816.1 |
Application
PTN Antibody (C-term) western blot analysis in U251,NCI-H460 cell line lysates (35ug/lane).This demonstrates the PTN antibody detected the PTN protein (arrow).
Application
PTN Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PTN Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
