Application
PTPRC Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PTPRC antibody detected the PTPRC protein (arrow).Application
PTPRC Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PTPRC Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD45 |
|---|---|
| Alternative Names | PTPRC CD45 Receptor-type tyrosine-protein phosphatase C Leukocyte common antigen T200 CD_antigen=CD45 Flags: Precursor |
| Molecular Weight(MW) | 147254 Da |
| Function | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN Dephosphorylates LYN, and thereby modulates LYN activity (By similarity) |
| Tissue Specificity | This PTPRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-373 amino acids from the N-terminal region of human PTPRC. |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts |
| Entrez Gene | 5788 |
|---|---|
| Nucleotide Accession | NP_563578.1 NP_002829.2 |
Application
PTPRC Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PTPRC antibody detected the PTPRC protein (arrow).
Application
PTPRC Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
