Application
Western blot analysis of PTTG1 antibody (N-term) (Cat.# AP7354a) in Ramos cell line lysates (35ug/lane). PTTG1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human testis tissue with PTTG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PTTG1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EAP1, PTTG, TUTR1 |
|---|---|
| Alternative Names | PTTG1 EAP1 PTTG TUTR1 Securin Esp1-associated protein Pituitary tumor-transforming gene 1 protein |
| Molecular Weight(MW) | 22024 Da |
| Function | Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53 The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation |
| Tissue Specificity | Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 9232 |
|---|
Application
Western blot analysis of PTTG1 antibody (N-term) (Cat.# AP7354a) in Ramos cell line lysates (35ug/lane). PTTG1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human testis tissue with PTTG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
