Application
PUS1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PUS1 antibody detected the PUS1 protein (arrow).Application
PUS1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PUS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PUS1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PUS1 tRNA pseudouridine synthase A mitochondrial tRNA pseudouridine(38-40) synthase tRNA pseudouridylate synthase I tRNA-uridine isomerase I |
|---|---|
| Molecular Weight(MW) | 47470 Da |
| Function | Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA (By similarity) |
| Tissue Specificity | Widely expressed. High levels of expression found in brain and skeletal muscle |
| Cellular Localization | Isoform 1: Mitochondrion. |
| Entrez Gene | 80324 |
|---|---|
| Nucleotide Accession | NP_001002019.1 NP_079491.2 |
Application
PUS1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PUS1 antibody detected the PUS1 protein (arrow).
Application
PUS1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PUS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
