Application
PYCR1 Antibody (C-term) western blot analysis in K562,WiDr,HepG2 cell line lysates (35ug/lane).This demonstrates the PYCR1 antibody detected the PYCR1 protein (arrow).Application
PYCR1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PYCR1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PYCR1 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PYCR1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PYCR1 Pyrroline-5-carboxylate reductase 1 mitochondrial |
|---|---|
| Molecular Weight(MW) | 33361 Da |
| Function | Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress |
| Tissue Specificity | This PYCR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290~319 amino acids from the C-terminal region of human PYCR1. |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 5831 |
|---|
Application
PYCR1 Antibody (C-term) western blot analysis in K562,WiDr,HepG2 cell line lysates (35ug/lane).This demonstrates the PYCR1 antibody detected the PYCR1 protein (arrow).
Application
PYCR1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PYCR1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PYCR1 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
