Application
Western blot analysis of PYGM (arrow) using rabbit polyclonal PYGM Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PYGM Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PYGM; Glycogen phosphorylase, muscle form; Myophosphorylase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PYGM Glycogen phosphorylase muscle form Myophosphorylase |
|---|---|
| Molecular Weight(MW) | 97092 Da |
| Function | Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates However, all known phosphorylases share catalytic and structural properties |
| Tissue Specificity | This PYGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705~735 amino acids from the C-terminal region of human PYGM. |
| Entrez Gene | 5837 |
|---|
Application
Western blot analysis of PYGM (arrow) using rabbit polyclonal PYGM Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
