Application
QTRTD1 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the QTRTD1 antibody detected the QTRTD1 protein (arrow).Application
QTRTD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of QTRTD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of QTRTD1 Antibody (C-term)(Cat#AP11696b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
QTRTD1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | QTRTD1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | QTRTD1; Queuine tRNA-ribosyltransferase subunit QTRTD1; Queuine tRNA-ribosyltransferase domain-containing protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | QTRTD1 Queuine tRNA-ribosyltransferase subunit QTRTD1 Queuine tRNA-ribosyltransferase domain-containing protein 1 |
|---|---|
| Molecular Weight(MW) | 46713 Da |
| Function | Interacts with QTRT1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine) (By similarity) |
| Tissue Specificity | This QTRTD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 328-359 amino acids from the C-terminal region of human QTRTD1. |
| Cellular Localization | Cytoplasm (By similarity). Mitochondrion (By similarity). Note=May associate with the mitochondrion outer membrane (By similarity) |
| Entrez Gene | 79691 |
|---|---|
| Nucleotide Accession | NP_078914.1 |
Application
QTRTD1 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the QTRTD1 antibody detected the QTRTD1 protein (arrow).
Application
QTRTD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of QTRTD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of QTRTD1 Antibody (C-term)(Cat#AP11696b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
QTRTD1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
